Sarcoma osteogenic-like Saos-2 cells on the silica nanofibres coated with nanodiamondPart of:
Marie Steinerova1, Elena Filova1, Alexander Kromka2, Maria Domonkos2, Lucie Bacakova1
1. Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic
2. Department of Optical Materials, Institute of Physics of the Czech Academy of Sciences, Cukrovarnicka 10, 162 00 Prague, Czech Republic
Nanotechnologies are widely used in bone tissue engineering. Nanodiamond materials have excellent biomechanical properties and can be modified to obtain biomimetic properties. The aim of the study was to prepare silica nano-fibres coated with nanodiamond and to characterise them in vitro in terms of their biocompatibility and bioactivity.
Saos-2 cells were cultured on SiOx materials covered with nanodiamond for 7 days. Materials were as follows: SiOx nano-fibres covered with nanodiamond by chemical vapour deposition (CVD) (H-termination), SiOx nano-fibres with nanodiamond after plasma O-termination, and SiOx nano-fibres without nanodiamond coating. As a control, glass coverslips were used. On the days 1, 4, and 7 after seeding, cell number and cell area on each of material was evaluated. The cells were also stained for proteins involved in osteoblasts adhesion, i.e. ?-actin (on the day 1) and vinculin (on the day 4), and in osteogenic differentiation, i.e. osteocalcin (on the day 7). On the day 7 cell metabolic activity (by resazurin assay) and ALP activity of the cells was measured as well. The concentration of vinculin and osteocalcin are being measured by enzymatic immunosorbent assay in cell homogenates prepared on day 7 after seeding.
The silica nanofibres coated with both H- and O-terminated nanodiamond supported the adhesion and proliferation of Saos-2 cells. These materials seem to be biocompatible and suitable for bone tissue engineering.
Supported by the AZV CR grant agency, project No. 15-33018A and the Grant Agency of the Czech Republic, project No. P108/12/G108.