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Poster Presentation

Sarcoma osteogenic-like Saos-2 cells on the silica nanofibres coated with nanodiamond

Sunday (01.01.2040)
00:00 - 08:01
Part of:
Line-Up:
- Poster Presentation The European user access program nanoscience foundries and fine analysis (NFFA) 0 Dr.-Ing. Thomas F. Keller
- Poster Presentation Sarcoma osteogenic-like Saos-2 cells on the silica nanofibres coated with nanodiamond 1 Marie Steinerova
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- Poster Presentation Development and Characterization of Hybrid Hydrogel Based on Oxidized Alginate and Keratin for Tissue Engineering Applications 0 Supachai Reakasame
- Poster Presentation Nir Laser-responsive Folate-targeted Gold Nanorods As Efficient theranostic tool for Osteosarcoma Treatment 1 Ph.D. Nicol? Mauro
- Poster Presentation Mechanical properties of recent CAD/CAM polymer materials 1 Sebastian Spintzyk
- Poster Presentation Multilayers of Nanoparticles with Alginate, Chondroitin sulfate and Hyaluronic acid for graded implants 1 Steffen Sydow
- Poster Presentation Design of drug eluting ophthalmic lenses through a multi-layer approach 1 Dr. Ana Topete
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- Poster Presentation Investigating the compressibility of spray dried alumina-zirconia granules for use in orthopaedics - an easy to use and fast method 1 Dr. Ranko Adjiski
- Poster Presentation Biochemical modification of extruded protein nanofibers 1 Karsten Stapelfeldt
- Poster Presentation Microfibers and Nanoparticles with Controlled Dimensions of a Hyaluronic Acid Derivative 1 Ph.D. Calogero Fiorica
- Poster Presentation Modeling the Viscosity of Silicone Rubber During High-Speed Infrared Curing 1 Jan Stieghorst
- Poster Presentation Effect of the pH of bleaching H2O2 solutions in the enamel morphology, hardness and color 1 Dr. Ana Topete
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- Poster Presentation Cellular interactions with extruded protein nanofibers 1 Naiana Suter
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- Poster Presentation Corrosion behavior of dental CoCr alloys made by different digital workflow processes compared to cast CoCr alloys 0 Christine Schille
- Poster Presentation Mechanical properties of biocompatible polymer-based resin for dental applications fabricated by stereolithography 1 Phan Hai-Binh Bui

Session P.2: Poster Session 2
Belongs to:
Topic P: Postersession


Marie Steinerova1, Elena Filova1, Alexander Kromka2, Maria Domonkos2, Lucie Bacakova1

1. Department of Biomaterials and Tissue Engineering, Institute of Physiology of the  Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech Republic

2. Department of Optical Materials, Institute of Physics of the Czech Academy of Sciences, Cukrovarnicka 10, 162 00 Prague, Czech Republic

Nanotechnologies are widely used in bone tissue engineering. Nanodiamond materials have excellent biomechanical properties and can be modified to obtain biomimetic properties. The aim of the study was to prepare silica nano-fibres coated with nanodiamond and to characterise them in vitro in terms of their biocompatibility and bioactivity. 

Saos-2 cells were cultured on SiOx materials covered with nanodiamond for 7 days.  Materials were as follows: SiOx nano-fibres covered with nanodiamond by chemical vapour deposition (CVD) (H-termination), SiOx nano-fibres with nanodiamond after plasma O-termination, and SiOx nano-fibres without nanodiamond coating. As a control, glass coverslips were used. On the days 1, 4, and 7 after seeding, cell number and cell area on each of material was evaluated. The cells were also stained for proteins involved in osteoblasts adhesion, i.e. ?-actin (on the day 1) and vinculin (on the day 4), and in osteogenic differentiation, i.e. osteocalcin (on the day 7). On the day 7 cell metabolic activity (by resazurin assay) and ALP activity of the cells was measured as well. The concentration of vinculin and osteocalcin are being measured by enzymatic immunosorbent assay in cell homogenates prepared on day 7 after seeding.

The silica nanofibres coated with both H- and O-terminated nanodiamond supported the adhesion and proliferation of Saos-2 cells. These materials seem to be biocompatible and suitable for bone tissue engineering.

Supported by the AZV CR grant agency, project No. 15-33018A and the Grant Agency of the Czech Republic, project No. P108/12/G108. 

 

 

Speaker:
Marie Steinerova
Czech Academy of Sciences
Additional Authors:
  • Elena Filova
    Czech Academy of Sciences
  • Alexander Kromka
    Czech Academy of Sciences
  • Maria Domonkos
    Czech Academy of Sciences
  • Lucie Bacakova
    Czech Academy of Sciences