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Influence of nanoripple patterns on stem cell adhesion and AFM manipulation experiments on glasses

Tuesday (09.05.2017)
12:20 - 12:40 Room Goethe III
Part of:

Enrico Gnecco(1), Angel Ayuso Sacido(2), Francesco Buatier de Mongeot(3)



Otto Schott Institute of Materials Research, Friedrich Schiller University

Jena, Germany



IMDEA Nanociencia & Hospital de Madrid Foundation, Madrid, Spain



Department of Physics, University of Genova, Italy


Rippled glass surfaces with characteristic

lengths in the 100 nm range are interesting benchmarks for investigating the

mobility of tiny objects using atomic force microscopy. In the first part of the

talk I will discuss recent nanomanipulation experiments on gold particles on

such substrates, as produced by defocused ion beam sputtering. Independently of

the direction of the mechanical forces applied by the probing tip, the

particles are “channeled” parallel to the ripples in a stick-slip fashion [1].

Similar patterns have been also used as substrates for mouse neural stem cells

(C17.2). In such case a considerable reduction in cell adhesion was observed,

as compared to flat glass substrates, which made impossible any attempts of

manipulating the cells without detaching them. On the other side, AFM images

proved that the cells kept an overall compact shape on the patterned

substrates, with their filopodia trapped parallel or perpendicular to the

ripples. This is completely different from the spreading of the cells observed

on flat glass substrates, where the filopodia are oriented in all directions

and also on the mechanical response observed on patterns with longer

characteristic length scales [2].




[1]          E.

Gnecco, P. Nita, S. Casado, C. Pimentel, K. Mougin, M.C. Giordano, D. Repetto

and F. Buatier de Mongeot, “Channeling motion of gold nanospheres on a rippled

glassed surface”, Nanotechnology 25 (2014) 485302


[2]          P.

Pedraz, S. Casado, V. Rodriguez, M.C. Giordano, F. Buatier de Mongeot, A. Ayuso

Sacido, and E. Gnecco, “Adhesion modification of neural stem cells induced by

nanoscale ripple patterns”, Nanotechnology 27 (2016) 125301




Prof. Dr. Enrico Gnecco
Friedrich Schiller University Jena