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Oral Poster

Effects of cross-linking modifications on Collagen: a preliminary biological evaluation

Tuesday (09.05.2017)
18:42 - 18:45
Part of:
18:00 Oral Poster Fibrinogen Adsorption and Network Formation on Melt Drawn Isotactic Polybutene-1 Thin Films 1 Xiaoyuan Zhang
18:03 Oral Poster Characterization of biomaterials using AFM based fast nanoscale imaging and quantitative nanomechanical techniques 1 Dr. Tanja Neumann
18:06 Oral Poster Hydrothermal conversion of porous Ca carbonate biominerals into bioactive Ca phosphate materials: towards antibiotic and antiosteoporotic bone graft substitutes 1 Dr. Ingo Sethmann
18:09 Oral Poster Study on the retarding effect of phytic acid on the hydration of a brushite cement 1 Dr. Katrin Hurle
18:12 Oral Poster Synthesis of hollow mesoporous bioactive glass nanoparticles with uniform size and shape 1 Kai Zheng
18:15 Oral Poster Systematic post-operative clinical assessment of lumbar ventrolateral vertebroplasty in the large animal model sheep using a minimally-invasive approach 1 Francesca Gunnella
18:18 Oral Poster Dual setting system – characterization of PEG-based-hydrogel/brushite composites for biomedical application 1 Michaela Rödel
18:21 Oral Poster The effect of HAc etching on the degradation behaviour of Mg-5Gd 1 Dr. Frank Feyerabend
18:24 Oral Poster Degradation behavior of as cast and powder metallurgy processed Mg-Ca alloys 0 Dipl.-Ing. Eshwara Phani Shubhakar Nidadavolu
18:27 Oral Poster Bio-inspired high-performance green biopolymer fibers 1 Dr. Hendrik Bargel
18:30 Oral Poster Diatom Biosilica for Catalytic and Adsorptive Applications 1 Cathleen Fischer
18:33 Oral Poster Gallotannin polyphenol enhancement of biomimetic enzymatic hydrogel mineralization 1 Dr. Timothy E.L. Douglas
18:36 Oral Poster Starch in textile application: Modification, processing and product development 1 Pavan Kumar Manvi
18:39 Oral Poster Recombinant Spider Silk Proteins Used as Biomedical Coating Material 1 Christian Borkner
18:42 Oral Poster Effects of cross-linking modifications on Collagen: a preliminary biological evaluation 1 Dr. Mattia Vacchini
18:45 Oral Poster Endothelialization of polymethylpentene fibers as a step towards patient-specific medical devices 1 M.Sc. Annika Wenz
18:48 Oral Poster Hybrid poly-lactide/gelatin electrospun scaffolds with controlled hydrophilicity and wettability. 1 Germano Piccirillo
18:51 Oral Poster Funktion and structure - Combined optical functionality and specific bio-interaction for multifunctional biomedical materials 1 Dr. Tina Sabel
18:57 Oral Poster Bacterial adhesion to PEEK compared to exemplary dental ceramic, metallic and composite materials 1 Dr. Lutz Scheideler
19:00 Oral Poster Attachment of Nanoparticle Drug-Release Systems on Shish-Kebab structured Poly(?-Caprolactone) Nanofibers 1 Dominik de Cassan
19:06 Oral Poster Improved Nanofleeces for Cell Culture Applications 1 Tobias Kürbitz

Session P.1: Oral Poster Session 1
Belongs to:
Topic P: Postersession

Claudio Bini, Roberto Guizzardi, Michela Ceriani, Costanza Trinchero,

Alessia D’Aloia, Stucchi Simone,  Barbara

Costa, Laura Cipolla, Mattia Vacchini*.


*Presenting author


Department of Biotechnology and Biosciences,

University of Milano-Bicocca , P.zza della Scienza 2, I-20126 Milano,


Along with

biomedicine development, tissue engineering is becoming a more and more

promising area of research, with ample room to find intelligent solutions to a

variety of clinical problems in the form of biocompatible and biodegradable

materials for tissue regeneration. In this area, the repair of cartilage defects

is a challenging and valuable topic which needs to be studied in detail to

fully exploit its potential.[1] Collagen-based scaffolds are

appealing products for the repair of cartilage defects. However, collagen has

low biomechanical stiffness and undergoes rapid biodegradation and biomaterials

based on it need to be stabilized.[2] This is achieved mainly by chemical

crosslinking methods, to obtained the desired mechanical properties for

implantation and defect repair. Several chemical agents have been used to

achieve this goal.[1] In this study we aimed to test a cross-linked

collagen obtained via a Methylglyoxal (MeG) reaction as chondrocytes behavioral

modulator. MeG is a physiological metabolite formed by several catabolic processes,

it is significantly present in the body and related to some pathological states.[3]

Beside to MeG reaction, cross-linking using 1,4-Butanediol diglycidyl ether

(BDDGE) is performed, to include a commonly used homobifunctional epoxide

compound to cross-link molecules as positive control.[4]

Cross-linked collagen, with altered physical and chemical properties, along

with unmodified one, is used to study the behavior of human chondrocyte C28 immortalized cell line,

focusing on growth, apoptosis and metalloproteinases expression as stress

indicator. BDDGE cross-link shows little improvements if compared to untreated

collagen; notably, MeG cross-linked collagen displays the highest growth and

lowest apoptosis rate in comparison to unmodified and BDDGE ones. Considering

metalloproteinases, unmodified collagen shows the highest expression while the

others exhibit comparable data. As MeG cross-linked collagen improves

chondrocyte C28 growth, further studies are in due course.


Acknowledgments. We gratefully acknowledge FA 2015 for

financial support.






1. Angele P. et al. Influence

of different collagen species on physico-chemical properties of crosslinked

collagen matrices. Biomaterials 25 (2004) 2831–2841


2. Lee CR. et al. The effects

of cross-linking of collagen-glycosaminoglycan scaffolds on compressive

stiffness, chondrocyte-mediated contraction, proliferation and biosynthesis.

Biomaterials 2001;22:3145–54.


3. Naoko Murata-Kamiya and

Hiroyuki Kamiya. Methylglyoxal, an endogenous aldehyde, crosslinks DNA

polymerase and the substrate DNA. Nucleic Acids Research, 2001, Vol. 29, No. 16



4. Hermanson GT Bioconjugate

Techniques, 2013.


Dr. Mattia Vacchini
University of Milano-Bicocca