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Glycoconjugation of Polycaprolactone (PCL) Nanostructured 2D Scaffolds

Tuesday (09.05.2017)
11:40 - 12:00 Room Goethe III
Part of:

Roberto Guizzardi,1 Marzia Lecchi,1 Valentina

Pastori,1 Chiara Battocchio,2 Valeria Secchi,2

Laura Cipolla1


1Department of Biotechnology and

Biosciences, University of Milano-Bicocca , P.zza della Scienza 2, I-20126

Milano, Italy.


2Department of Sciences, INSTM, CNISM and

CISDiC, University Roma Tre, Via della Vasca Navale 84, 00146 Rome, Italy.





materials, in which the material scaffold has been functionalized with biomolecules

in order to promote the desired biological response, increase the chances of

tissue regeneration and wound healing.


Cell adhesion and

differentiation is strongly related to the nature of biomaterial and the physico-chemical

surface properties such as charge, wettability or the presence of bioactive

compounds. Several classes of biomolecules, such as proteins, lipids and

carbohydrates, are known to enhance specific cellular response, and have been

exploited in recent years for the bio-activation of material surfaces. In this

field, glycans have already shown their promising role in tissue engineering

applications.1 It is now well established that glycan functionalized

biomaterials represent a new class of nanostructured materials able to guide and

control cell-behaviour in regenerative medicine strategies. Among synthetic

polymers, aliphatic polyesters such as poly(?-caprolactone) (PCL) have been

widely considered as biomaterials for designing scaffolds to support the

regeneration of several tissues, due to a unique combination of

biodegradability and biocompatibility properties. From these premises,

glycoconjugation of PLC-based materials is desired in order to obtain new smart

biomaterials able to favour regenerative processes through the control and the

modulation of cell adhesion, differentiation and proliferation.3

Here we propose the synthesis and characterization  of glycosylated PCL by two-step procedure. The

successfulness of PCL surface modification was probed by means of X-ray

Photoemission Spectroscopy (XPS) and Near Edge X-ray Absorption Fine Structure

(NEXAFS) spectroscopy.



biological evaluation in terms of cytocompatibility have also been performed.




Acknowledgments. We gratefully acknowledge FA 2015 for

financial support.









Russo, L.; Battocchio, C.; Secchi, V.; Magnano, E.; Nappini, S.; Taraballi, F.;

Gabrielli, L.; Comelli, F.; Papagni, A.; Costa, B.; Polzonetti, G.; Nicotra,

F.; Natalello, A.; Doglia, S. M.; Cipolla, L. Langmuir 2014, 30,




Russo, L.; Sgambato, A.; Lecchi, M.; Pastori, V.; Raspanti, M.; Natalello, A.;

Doglia, S.M.; Nicotra, F.; Cipolla, L. ACS Chem. Neurosci. 2014, 5,




Russo, L.; Gloria A.; Russo T.; D'Amora, U.; Taraballi F.; De Santis, R.;

Ambrosio, L.; Nicotra, F.; Cipolla L. RSC Advances 2013, 3, 6286. 




Dr. Roberto Guizzardi
University of Milano-Bicocca
Additional Authors:
  • Marzia Lecchi
    Universit? degli Studi di Milano-Bicocca
  • Valentina Pastori
    Universit? degli Studi di Milano-Bicocca
  • Prof. Chiara Battocchio
    Universit? Roma Tre
  • Valeria Secchi
    Universit? Roma Tre
  • Dr. Laura Cipolla
    Universit? degli Studi di Milano-Bicocca