Lecture
Glycoconjugation of Polycaprolactone (PCL) Nanostructured 2D Scaffolds
Tuesday (09.05.2017) 11:40 - 12:00 Room Goethe III Part of:Roberto Guizzardi,1 Marzia Lecchi,1 Valentina
Pastori,1 Chiara Battocchio,2 Valeria Secchi,2
Laura Cipolla1
1Department of Biotechnology and
Biosciences, University of Milano-Bicocca , P.zza della Scienza 2, I-20126
Milano, Italy.
2Department of Sciences, INSTM, CNISM and
CISDiC, University Roma Tre, Via della Vasca Navale 84, 00146 Rome, Italy.
Bioactive
materials, in which the material scaffold has been functionalized with biomolecules
in order to promote the desired biological response, increase the chances of
tissue regeneration and wound healing.
Cell adhesion and
differentiation is strongly related to the nature of biomaterial and the physico-chemical
surface properties such as charge, wettability or the presence of bioactive
compounds. Several classes of biomolecules, such as proteins, lipids and
carbohydrates, are known to enhance specific cellular response, and have been
exploited in recent years for the bio-activation of material surfaces. In this
field, glycans have already shown their promising role in tissue engineering
applications.1 It is now well established that glycan functionalized
biomaterials represent a new class of nanostructured materials able to guide and
control cell-behaviour in regenerative medicine strategies. Among synthetic
polymers, aliphatic polyesters such as poly(?-caprolactone) (PCL) have been
widely considered as biomaterials for designing scaffolds to support the
regeneration of several tissues, due to a unique combination of
biodegradability and biocompatibility properties. From these premises,
glycoconjugation of PLC-based materials is desired in order to obtain new smart
biomaterials able to favour regenerative processes through the control and the
modulation of cell adhesion, differentiation and proliferation.3
Here we propose the synthesis and characterization of glycosylated PCL by two-step procedure. The
successfulness of PCL surface modification was probed by means of X-ray
Photoemission Spectroscopy (XPS) and Near Edge X-ray Absorption Fine Structure
(NEXAFS) spectroscopy.
Preliminary
biological evaluation in terms of cytocompatibility have also been performed.
Acknowledgments. We gratefully acknowledge FA 2015 for
financial support.
References
[1]
Russo, L.; Battocchio, C.; Secchi, V.; Magnano, E.; Nappini, S.; Taraballi, F.;
Gabrielli, L.; Comelli, F.; Papagni, A.; Costa, B.; Polzonetti, G.; Nicotra,
F.; Natalello, A.; Doglia, S. M.; Cipolla, L. Langmuir 2014, 30,
1336;
[2]
Russo, L.; Sgambato, A.; Lecchi, M.; Pastori, V.; Raspanti, M.; Natalello, A.;
Doglia, S.M.; Nicotra, F.; Cipolla, L. ACS Chem. Neurosci. 2014, 5,
261.
[3]
Russo, L.; Gloria A.; Russo T.; D'Amora, U.; Taraballi F.; De Santis, R.;
Ambrosio, L.; Nicotra, F.; Cipolla L. RSC Advances 2013, 3, 6286.
