Interface tissue engineering aims to repair or promote the neo-tissue formation in diseased areas between different tissues types . The electrospinning is a scaffold fabrication technique for the fabrication of nano or microsized fibrous mats. The versatility of this technique allows the fabrication of multilayered scaffolds, by sequential process deposition, obtaining different layers with gradient in composition (chemical gradient), by the incorporation of inorganic particles inside the electrospun fibers and morphological gradient (porosity gradient). In the present work, the added value in the processing for the obtainment of these multilayered scaffolds is represented by the use of benign solvents for the electrospinning. Since the definition of “Green Electrospinning” in 2010 , the interest in the use of environmental friendly solvents for the electrospinning, in particular for biomedical applications, increased in the last years.
In the present work, bilayered electrospun mats with gradient in composition and porosity were fabricated. In particular, the addition of bioactive glass particles was investigated for the obtainment of a bioactive layer, reporting a selective bioactivity only on the composite layer and absence of any deposits on the upper polymeric layer.
These multilayered electrospun scaffolds were also used in combination with foam replica method technique for the obtainment of multilayered scaffolds for osteochondral tissue engineering applications, by exploiting the proof of concept already reported in literature .
Positive and promising results were obtained and reported after morphological, chemical, mechanical and acellular bioactivity characterizations.
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