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Oral Poster

Characterization of biomaterials using AFM based fast nanoscale imaging and quantitative nanomechanical techniques

Tuesday (09.05.2017)
18:03 - 18:06
Part of:
18:00 Oral Poster Fibrinogen Adsorption and Network Formation on Melt Drawn Isotactic Polybutene-1 Thin Films 1 Xiaoyuan Zhang
18:03 Oral Poster Characterization of biomaterials using AFM based fast nanoscale imaging and quantitative nanomechanical techniques 1 Dr. Tanja Neumann
18:06 Oral Poster Hydrothermal conversion of porous Ca carbonate biominerals into bioactive Ca phosphate materials: towards antibiotic and antiosteoporotic bone graft substitutes 1 Dr. Ingo Sethmann
18:09 Oral Poster Study on the retarding effect of phytic acid on the hydration of a brushite cement 1 Dr. Katrin Hurle
18:12 Oral Poster Synthesis of hollow mesoporous bioactive glass nanoparticles with uniform size and shape 1 Kai Zheng
18:15 Oral Poster Systematic post-operative clinical assessment of lumbar ventrolateral vertebroplasty in the large animal model sheep using a minimally-invasive approach 1 Francesca Gunnella
18:18 Oral Poster Dual setting system – characterization of PEG-based-hydrogel/brushite composites for biomedical application 1 Michaela Rödel
18:21 Oral Poster The effect of HAc etching on the degradation behaviour of Mg-5Gd 1 Dr. Frank Feyerabend
18:24 Oral Poster Degradation behavior of as cast and powder metallurgy processed Mg-Ca alloys 0 Dipl.-Ing. Eshwara Phani Shubhakar Nidadavolu
18:27 Oral Poster Bio-inspired high-performance green biopolymer fibers 1 Dr. Hendrik Bargel
18:30 Oral Poster Diatom Biosilica for Catalytic and Adsorptive Applications 1 Cathleen Fischer
18:33 Oral Poster Gallotannin polyphenol enhancement of biomimetic enzymatic hydrogel mineralization 1 Dr. Timothy E.L. Douglas
18:36 Oral Poster Starch in textile application: Modification, processing and product development 1 Pavan Kumar Manvi
18:39 Oral Poster Recombinant Spider Silk Proteins Used as Biomedical Coating Material 1 Christian Borkner
18:42 Oral Poster Effects of cross-linking modifications on Collagen: a preliminary biological evaluation 1 Dr. Mattia Vacchini
18:45 Oral Poster Endothelialization of polymethylpentene fibers as a step towards patient-specific medical devices 1 M.Sc. Annika Wenz
18:48 Oral Poster Hybrid poly-lactide/gelatin electrospun scaffolds with controlled hydrophilicity and wettability. 1 Germano Piccirillo
18:51 Oral Poster Funktion and structure - Combined optical functionality and specific bio-interaction for multifunctional biomedical materials 1 Dr. Tina Sabel
18:57 Oral Poster Bacterial adhesion to PEEK compared to exemplary dental ceramic, metallic and composite materials 1 Dr. Lutz Scheideler
19:00 Oral Poster Attachment of Nanoparticle Drug-Release Systems on Shish-Kebab structured Poly(?-Caprolactone) Nanofibers 1 Dominik de Cassan
19:06 Oral Poster Improved Nanofleeces for Cell Culture Applications 1 Tobias Kürbitz

Session P.1: Oral Poster Session 1
Belongs to:
Topic P: Postersession

Besides structural and physico-chemical composition, topography, roughness, adhesiveness as well as mechanical properties of biomaterials are relevant factors making them suitable for biomedical applications. All these factors affect cell differentiation and tissue formation, and are crucial for their integration as well as healing capacity in the human body. Atomic Force Microscopy is suitable for measuring all of these characteristics with nanometer scale resolution under physiological conditions. We have developed a multipurpose AFM device allowing comprehensive characterization of biological samples such as live cells, tissues and biomaterials in the nanoscale. True optical integration allows the simultaneous use of advanced inverted optical microscope techniques such as DIC or confocal laser scanning microscopy, but also upright optics, such as macroscopes for the investigation of opaque samples.

With our “Quantitative Imaging” (QI™) mode several sample properties, such as the topography, stiffness and adhesiveness, can be obtained with one measurement in high resolution. Even more complex data like Young´s modulus images, topography at different indentation forces in terms of tomography, or recognition events can be obtained. A variety of biological samples have been investigated to demonstrate the capability and flexibility of QI™. The NanoWizard® ULTRA Speed technique allows fast AFM imaging of dynamic processes with approximately 1 frame per second. The kinetics of collagen type I fibrillogenesis was imaged in situ with high spatiotemporal resolution, revealing the formation of the 67 nm D-banding hallmark. With the CellHesion® technique, the adhesion of a single living cell to any substrate can be measured and validated using comprehensive analysis tools. The Side-view cantilever holder enables a side view of the cell-sample interface while performing adhesion experiments, providing complementary information without expensive z-stacking.

The inherent drawbacks of traditional AFM imaging modes for fast imaging or for challenging samples like living cells can be overcome by the NanoWizard® ULTRA Speed and QI™ mode. We present an enhancement of AFM technique providing a versatile tool for an extensive characterization of biomaterials.

Dr. Tanja Neumann
Bruker Nano GmbH
Additional Authors:
  • Dr. Torsten Müller
    JPK Instruments AG
  • Dr. Dimitar Stamov
    JPK Instruments AG
  • Heiko Haschke
    JPK Instruments AG
  • Sandra Kostrowski
    JPK Instruments AG
  • Carmen Petterson
    JPK Instruments AG
  • Torsten Jähnke
    JPK Instruments AG