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Oral Poster

Gallotannin polyphenol enhancement of biomimetic enzymatic hydrogel mineralization

Tuesday (09.05.2017)
18:33 - 18:36
Part of:
18:00 Oral Poster Fibrinogen Adsorption and Network Formation on Melt Drawn Isotactic Polybutene-1 Thin Films 1 Xiaoyuan Zhang
18:03 Oral Poster Characterization of biomaterials using AFM based fast nanoscale imaging and quantitative nanomechanical techniques 1 Dr. Tanja Neumann
18:06 Oral Poster Hydrothermal conversion of porous Ca carbonate biominerals into bioactive Ca phosphate materials: towards antibiotic and antiosteoporotic bone graft substitutes 1 Dr. Ingo Sethmann
18:09 Oral Poster Study on the retarding effect of phytic acid on the hydration of a brushite cement 1 Dr. Katrin Hurle
18:12 Oral Poster Synthesis of hollow mesoporous bioactive glass nanoparticles with uniform size and shape 1 Kai Zheng
18:15 Oral Poster Systematic post-operative clinical assessment of lumbar ventrolateral vertebroplasty in the large animal model sheep using a minimally-invasive approach 1 Francesca Gunnella
18:18 Oral Poster Dual setting system – characterization of PEG-based-hydrogel/brushite composites for biomedical application 1 Michaela Rödel
18:21 Oral Poster The effect of HAc etching on the degradation behaviour of Mg-5Gd 1 Dr. Frank Feyerabend
18:24 Oral Poster Degradation behavior of as cast and powder metallurgy processed Mg-Ca alloys 0 Dipl.-Ing. Eshwara Phani Shubhakar Nidadavolu
18:27 Oral Poster Bio-inspired high-performance green biopolymer fibers 1 Dr. Hendrik Bargel
18:30 Oral Poster Diatom Biosilica for Catalytic and Adsorptive Applications 1 Cathleen Fischer
18:33 Oral Poster Gallotannin polyphenol enhancement of biomimetic enzymatic hydrogel mineralization 1 Dr. Timothy E.L. Douglas
18:36 Oral Poster Starch in textile application: Modification, processing and product development 1 Pavan Kumar Manvi
18:39 Oral Poster Recombinant Spider Silk Proteins Used as Biomedical Coating Material 1 Christian Borkner
18:42 Oral Poster Effects of cross-linking modifications on Collagen: a preliminary biological evaluation 1 Dr. Mattia Vacchini
18:45 Oral Poster Endothelialization of polymethylpentene fibers as a step towards patient-specific medical devices 1 M.Sc. Annika Wenz
18:48 Oral Poster Hybrid poly-lactide/gelatin electrospun scaffolds with controlled hydrophilicity and wettability. 1 Germano Piccirillo
18:51 Oral Poster Funktion and structure - Combined optical functionality and specific bio-interaction for multifunctional biomedical materials 1 Dr. Tina Sabel
18:57 Oral Poster Bacterial adhesion to PEEK compared to exemplary dental ceramic, metallic and composite materials 1 Dr. Lutz Scheideler
19:00 Oral Poster Attachment of Nanoparticle Drug-Release Systems on Shish-Kebab structured Poly(?-Caprolactone) Nanofibers 1 Dominik de Cassan
19:06 Oral Poster Improved Nanofleeces for Cell Culture Applications 1 Tobias Kürbitz

Session P.1: Oral Poster Session 1
Belongs to:
Topic P: Postersession

Marta Vandrovcová, Julia K. Keppler, Michelle Feuereisen, Patrick Ricquier, Andreas Schieber, Andre G. Skirtach, Timothy E.L. Douglas

Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered desirable to improve their suitability as materials for bone regeneration. One biomimetic strategy to achieve hydrogel mineralization involves incorporation of the enzyme responsible for mineralization of bone tissue, alkaline phosphatase (ALP), inside hydrogels, followed by incubation containing calcium ions and an organophosphate substrate for ALP, e.g. glycerophosphate (GP). ALP causes release of phosphate from GP, leading to elevated phosphate concentrations, which promote CaP precipitation. Another strategy is the incorporation of calcium-binding organic biomolecules inside hydrogels. This raises intrahydrogel calcium concentration, promoting CaP precipitation. Polyphenols are organic biomolecules consisting of phenolic units. Certain polyphenols have been reported to show affinity for calcium ions. A further advantage of using polyphenols is their antimicrobial activity, which can combat infection. In this study, the ability of different gallotannins (a class of polyphenols) to promote the ALP-mediated mineralizaton hydrogels of gellan gum (GG) was compared. ALP-loaded hydrogels were enriched with Pentagalloyl glucose (PGG), tannic acid preparations of differing molecular weights (1450 kD, 1060 kD and 850 kD) (all Ajinomoto OmniChem n.v., Wetteren, Belgium), and a gallotannin-rich extract from Mango kernel (Mangifera indica L.). All gallotannins and the mango extract promoted mineral formation. Mineralization was promoted most strongly by PGG, the mango extract and the high molecular weight tannic acid. All gallotannins and the mango extract interacted with ALP, as demonstrated by fluorescence quenching analysis. In addition, ALP aggregation was observed after addition of some of the gallotannons, especially for PGG (~800 nm diameter), as determined by dynamic light scattering. Further work will concentrate on the antimicrobial activity of the polyphenol-enriched mineralized hydrogels, as well as their cytocompatibility.

Additional Authors:
  • Dr. Marta Vandrovcová
    Czech Academy of Sciences
  • Dr. Julia Keppler
    Christian-Albrechts-Universität zu Kiel
  • Michelle Feuereisen
    Universität Bonn
  • Dr. Patrick Ricquier
    s.a. Ajinomoto OmniChem n.v.
  • Prof. Andreas Schieber
    Universität Bonn
  • Prof. Andre Skirtach
    Ghent University