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Oral Poster

Endothelialization of polymethylpentene fibers as a step towards patient-specific medical devices

Tuesday (09.05.2017)
18:45 - 18:48
Part of:
Line-Up:
18:00 Oral Poster Fibrinogen Adsorption and Network Formation on Melt Drawn Isotactic Polybutene-1 Thin Films 1 Xiaoyuan Zhang
18:03 Oral Poster Characterization of biomaterials using AFM based fast nanoscale imaging and quantitative nanomechanical techniques 1 Dr. Tanja Neumann
18:06 Oral Poster Hydrothermal conversion of porous Ca carbonate biominerals into bioactive Ca phosphate materials: towards antibiotic and antiosteoporotic bone graft substitutes 1 Dr. Ingo Sethmann
18:09 Oral Poster Study on the retarding effect of phytic acid on the hydration of a brushite cement 1 Dr. Katrin Hurle
18:12 Oral Poster Synthesis of hollow mesoporous bioactive glass nanoparticles with uniform size and shape 1 Kai Zheng
18:15 Oral Poster Systematic post-operative clinical assessment of lumbar ventrolateral vertebroplasty in the large animal model sheep using a minimally-invasive approach 1 Francesca Gunnella
18:18 Oral Poster Dual setting system – characterization of PEG-based-hydrogel/brushite composites for biomedical application 1 Michaela Rödel
18:21 Oral Poster The effect of HAc etching on the degradation behaviour of Mg-5Gd 1 Dr. Frank Feyerabend
18:24 Oral Poster Degradation behavior of as cast and powder metallurgy processed Mg-Ca alloys 0 Dipl.-Ing. Eshwara Phani Shubhakar Nidadavolu
18:27 Oral Poster Bio-inspired high-performance green biopolymer fibers 1 Dr. Hendrik Bargel
18:30 Oral Poster Diatom Biosilica for Catalytic and Adsorptive Applications 1 Cathleen Fischer
18:33 Oral Poster Gallotannin polyphenol enhancement of biomimetic enzymatic hydrogel mineralization 1 Dr. Timothy E.L. Douglas
18:36 Oral Poster Starch in textile application: Modification, processing and product development 1 Pavan Kumar Manvi
18:39 Oral Poster Recombinant Spider Silk Proteins Used as Biomedical Coating Material 1 Christian Borkner
18:42 Oral Poster Effects of cross-linking modifications on Collagen: a preliminary biological evaluation 1 Dr. Mattia Vacchini
18:45 Oral Poster Endothelialization of polymethylpentene fibers as a step towards patient-specific medical devices 1 M.Sc. Annika Wenz
18:48 Oral Poster Hybrid poly-lactide/gelatin electrospun scaffolds with controlled hydrophilicity and wettability. 1 Germano Piccirillo
18:51 Oral Poster Funktion and structure - Combined optical functionality and specific bio-interaction for multifunctional biomedical materials 1 Dr. Tina Sabel
18:57 Oral Poster Bacterial adhesion to PEEK compared to exemplary dental ceramic, metallic and composite materials 1 Dr. Lutz Scheideler
19:00 Oral Poster Attachment of Nanoparticle Drug-Release Systems on Shish-Kebab structured Poly(?-Caprolactone) Nanofibers 1 Dominik de Cassan
19:06 Oral Poster Improved Nanofleeces for Cell Culture Applications 1 Tobias Kürbitz

Session P.1: Oral Poster Session 1
Belongs to:
Topic P: Postersession


For the treatment of severe lung damage which occurs for example in patients with chronic obstructive lung disease, membrane oxygenators are used for oxygenation and decarboxylation of the patient’s blood. Seeding of polymethylpentene (PMP) membranes, were the gas exchange takes place, with an endothelial monolayer is supposed to improve hemocompatibility of the material and the gas exchanger’s permanency. To evaluate the potential of culturing human microvascular endothelial cells (ECs), seeding experiments were conducted on unmodified PMP membranes and the cells were characterized.


Primary ECs from human skin biopsies were seeded on unmodified PMP fibers and cultured in cell-specific medium for up to 7 days. Afterwards, the viability assessment of the cells via live-dead and MTT-staining showed a high viability of the cells, with only few dead cells found on the surfaces. Staining of EC-specific markers showed the identity and functionality of the cells. The cells formed cell-cell contacts which were demonstrated by the staining of PECAM-1 and VE-cadherin, verifying an intact monolayer which is essential for the mediation of hemocompatability. Further markers like van Willebrand factor (vWF) as well as functionality markers like the uptake of acetylated low density lipoprotein (acLDL) proved the identity of the cells.


In contrast to former studies, our results showed the successful culture of primary human ECs on PMP fibers without a prior surface modification. Important cell type specific markers could be detected and show that primary ECs seeded on the PMP fibers keep their identity as well as their functionality, a prerequisite for the formation of an intact endothelial monolayer and a first step towards the endothelialization of a gas exchanger’s membrane surface with patient-specific cells.

 

Speaker:
M.Sc. Annika Wenz
University of Stuttgart
Additional Authors:
  • Kirstin Linke
    Fraunhofer IGB, Stuttgart
  • Markus Schandar
    Fraunhofer IGB, Stuttgart
  • Dr. Esther Novosel
    Novalung, Heilbronn
  • Prof. Dr. Petra J. Kluger
    Reutlingen University; Fraunhofer IGB, Stuttgart