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Poster Presentation

Adjusted material-cell interaction using engineered spider silk proteins

Sunday (01.01.2040)
00:00 - 08:11
Part of:
Line-Up:
- Poster Presentation The European user access program nanoscience foundries and fine analysis (NFFA) 0 Dr.-Ing. Thomas F. Keller
- Poster Presentation Sarcoma osteogenic-like Saos-2 cells on the silica nanofibres coated with nanodiamond 1 Marie Steinerova
- Poster Presentation Sputtered, micro structured freestanding FeMn foils as biodegradable material 0 Till Jurgeleit
- Poster Presentation Exploring the effects of organic components on the degradation of magnesium in vitro under physiological conditions 1 Ph.D. Ruiqing Hou
- Poster Presentation Influence of laser treatment on the corrosion behavior of Mg-1Zn-1Ca and Mg-3Zn-0.3Ca alloys 1 Ph.D. Nuria Pulido González
- Poster Presentation PLA based CaCO3 composite fibers for textile scaffolds in hard tissue regeneration 1 Dr.-Ing. Claudia Hinüber
- Poster Presentation Functionalization of polymer fibers with heparan sulfate as a representative of the extracellular matrix 1 Inga Wille
- Poster Presentation Development and Characterization of Hybrid Hydrogel Based on Oxidized Alginate and Keratin for Tissue Engineering Applications 0 Supachai Reakasame
- Poster Presentation Nir Laser-responsive Folate-targeted Gold Nanorods As Efficient theranostic tool for Osteosarcoma Treatment 1 Ph.D. Nicol? Mauro
- Poster Presentation Mechanical properties of recent CAD/CAM polymer materials 1 Sebastian Spintzyk
- Poster Presentation Multilayers of Nanoparticles with Alginate, Chondroitin sulfate and Hyaluronic acid for graded implants 1 Steffen Sydow
- Poster Presentation Design of drug eluting ophthalmic lenses through a multi-layer approach 1 Dr. Ana Topete
- Poster Presentation Drug loaded silicon based soft contact lenses: effect of terminal sterilization methods on their performance 1 Andreia Sofia Oliveira
- Poster Presentation Investigating the compressibility of spray dried alumina-zirconia granules for use in orthopaedics - an easy to use and fast method 1 Dr. Ranko Adjiski
- Poster Presentation Biochemical modification of extruded protein nanofibers 1 Karsten Stapelfeldt
- Poster Presentation Microfibers and Nanoparticles with Controlled Dimensions of a Hyaluronic Acid Derivative 1 Ph.D. Calogero Fiorica
- Poster Presentation Modeling the Viscosity of Silicone Rubber During High-Speed Infrared Curing 1 Jan Stieghorst
- Poster Presentation Effect of the pH of bleaching H2O2 solutions in the enamel morphology, hardness and color 1 Dr. Ana Topete
- Poster Presentation New crosslinking diluents for dental materials 1 Prof. Dr. Norbert Moszner
- Poster Presentation Microstructure and Properties of Plasma Sprayed Bioactive Glass Ceramics Coatings 1 Annamária Dobrádi
- Poster Presentation Impact of chloride ions on apatite mineralisation on Bioglass® 45S5 0 Juliane Brandt-Slowik
- Poster Presentation In vivo performance of polymeric photodynamic active biomaterials for periodontal regeneration 1 Dr. Stefan Kranz
- Poster Presentation Adjusted material-cell interaction using engineered spider silk proteins 1 Vanessa Trossmann
- Poster Presentation Cellular interactions with extruded protein nanofibers 1 Naiana Suter
- Poster Presentation Antimicrobial efficacy of strontium and zinc glasses and glass ionomer cements against clinically relevant bacteria 0 Hawshan Mustafa
- Poster Presentation Mechanical properties of water-soluble polyvinyl alcohol parts fabricated by fused deposition modelling 1 Jochen Kuttig
- Poster Presentation Corrosion behavior of dental CoCr alloys made by different digital workflow processes compared to cast CoCr alloys 0 Christine Schille
- Poster Presentation Mechanical properties of biocompatible polymer-based resin for dental applications fabricated by stereolithography 1 Phan Hai-Binh Bui

Session P.2: Poster Session 2
Belongs to:
Topic P: Postersession


Spider silks are biodegradable, biocompatible, hypoallergenic, non-inflammatory and show extraordinary mechanical properties, including high elasticity and strength (1). Therefore, they combine interesting features for medical, clinical and pharmaceutical applications (2). A recombinant spider silk protein was designed and produced in a biotechnological process using E.coli to ensure generation of high amounts with consistent, reproducible properties (3,4). After encoding the amino acid composition of the dragline silk from Araneus diadematus, it was possible to develop naturally inspired, but recombinantly produced spider silk proteins, such as the 16 times repeated engineered Araneus diadematus fibroin 4, shortly eADF4(C16) (4). Engineered proteins exhibit similar properties to the naturally occurring spider silk, but also allow for high-yield production (4). As eADF4(C16) doesn’t contain specific cell adhesive motifs, cells, for example BALB/3T3 mouse fibroblasts, display low adhesion and proliferation on scaffolds made of these spider silk proteins (5). Thus, it is essential to adjust the cell binding properties by functionalizing the engineered spider silk protein eADF4(C16) to promote cell interaction (6-10). In this context, the recombinant silk protein eADF4(C16) was modified by genetically fusing the integrin recognition sequence RGD (7). The fusion protein eADF4(C16)-RGD showed similar properties to the unmodified spider silk protein with the exception of an improved cell attachment and proliferation as seen on RGD-silk films (7) or hydrogels (11). Although these results have shown the biomedical potential of these proteins, there is still room for further development in the future. In particular, the combination of cells and materials within tissue-like structures using one fabrication process, also known as biofabrication, is in our focus.

Speaker:
Vanessa Trossmann
University of Bayreuth
Additional Authors:
  • Elise DeSimone
    Universität Bayreuth
  • Prof. Dr. Thomas Scheibel
    Universität Bayreuth