Abstract – Human plasma fibrinogen (HPF) network formation is the key factor to initiate blood clotting or coagulation, which can be life-saving but also a severe complication when induced by artificial implant surfaces. Therefore, to understand and control HPF adsorption and network formation is of high importance. To create well-defined, repeatable and technically relevant polymer crystal surfaces, yet with distinct topographies, we rely a well-established morphology of semi-crystalline isotactic poly(butane-1) (iPB-1): shish-kebab crystals and needle-like crystals, by spin-coating and melt-drawing techniques, respectively. The adsorption behavior of HPF in dependence of two surface topographies were investigated by atomic force microscopy (AFM) and quartz crystal microbalance with dissipation (QCM-D). This study shows that the polymer crystal surface topography has a distinct effect on the HPF adsorption and its network formation. Increased number of HPF ring-like networks formed on needle-like crystals (NCs) as compared to the shish-kebab crystals (SKs). The control over the network formation of protein layers on material surfaces is essential for controlling the cell attachment. This understanding may provide a framework for extending the patterning approach to other proteins and material systems.
Keywords – Human plasma fibrinogen, Nanostructure, Polymer crystals, Isotactic Polybutene-1, Melt-drawing