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Lecture

Self-Assembled Protein Nanofibers as Basis for Novel Biomaterials

Wednesday (10.05.2017)
16:00 - 16:20 Room Bach
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Over

the last years, the interest in materials consisting of biomolecules arranged

in nanofibers increased. These materials have broad potential applications in

materials science, tissue engineering and biomedical engineering, for instance,

as scaffolds for cell cultures or drug delivery, as biosensors and as

functional templates for other materials. There is a special focus on plasma

proteins for applications in nanofiber materials because of their high

biocompatibility. An easy feasible strategy to create these nanofibers is the

self-assembly mechanism of protein molecules. Furthermore, the understanding of

this little-known mechanism is fundamental.

 

Here

we test the hypothesis that novel self-assembled hybrid protein nanofibers (PNNF)

can consist of two different proteins.

 

The PNNFs

were produced by incubating different plasma protein combinations in a mixture

of Millipore water and ethanol by elevated temperatures for various time

periods. For atomic force microscopy and tip enhanced raman spectroscopy measurements

the self-assembled PNNFs were dropped onto activated silicon as well as cleaned

and activated glass substrates. Mechanical and biological properties were

analysed.

 

In

this work we present, for the first time, self-assembled plasma PNNF in the

presence of a second protein. A novel PNNF hybrid will be presented too. Further,

long-time CD-measurements provide information about the fiber formation

dynamics. Especially, for the PNNF hybrid it confirmed interactions between

both molecules. Additionally, the influence of the second protein on the

properties of the novel hybrid PNNF is shown.

 

We

demonstrated the possibility to create self-assembled PNNFs in the presence of two

different plasma proteins. Further, we confirmed the existence of a novel PNF

hybrid. These results lay the foundation for a novel biomaterial based on these

PNF/PNF hybrids.

Speaker:
Christian Helbing
Friedrich Schiller University Jena
Additional Authors:
  • Dr. Tanja Deckert-Gaudig
    Leibniz Institute of Photonic Technology
  • Dr. Gang Wei
    University Bremen
  • Prof. Dr. Klaus D. Jandt
    Friedrich Schiller University Jena