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Reduced adhesion forces on nanoparticle-structured surfaces

Tuesday (09.05.2017)
15:10 - 15:30
Part of:


Biomaterials-associated infections

(BAI) are a widespread problem. Due to multi-resistant microorganisms, an

alternative and new antibiotic-free strategy to reduce the risk of a BAI is the

modification of the materials surface topography on the nanometer scale. Nanoparticles

are used for nanostructuring materials surfaces. Often only qualitative

adhesion properties are analyzed limiting the quantitative information about adhesion

forces between microbial cells and materials surfaces. An option focusing on

measuring adhesion forces is force-distance curves.




Therefore, the aims of

this study are [i] to investigate the adhesion of Candida albicans as function of materials surfaces physically

nanostructured with nanoparticles and [ii] to investigate force-distance curves

of C. albicans on these materials





Different gold nanoparticle

(AuNP) densities were immobilized on gold sputtered surfaces. The

nanostructured materials surfaces were characterized by atomic force microscopy

(AFM), contact angle measurements and X-ray photoelectron spectroscopy.

Microbial adhesion on these surfaces was investigated for different adhesion

times and adhesion kinetics were obtained using confocal laser scanning

microscopy. For quantitative analysis, microbial cells were attached on a

hollow AFM cantilever.




The investigated

surfaces with AuNPs densities of 25 ± 4 AuNP/µm2 and

61 ± 10 AuNP/µm2 as well as unstructured control

surfaces showed no statistically significant differences in contact angle and

surfaces chemistry. A reduced microbial adhesion was observed on the

nanostructured surfaces compared to the unstructured control surfaces. The AuNP

function as contact points for initial microbial adhesion which can be confirmed

by the results of the force-distance curves. A lower AuNP density led to a

reduced microbial adhesion as well as a reduced adhesion force between the

microbial cells and materials surfaces.



This study will provide new insight into microbial

adhesion on materials surfaces structured in the nanometer range. These

surfaces may have a potential for reducing microbial adhesion and the risk of



Carolin Dewald
Friedrich Schiller University Jena
Additional Authors:
  • Dr. Claudia Lüdecke
    Friedrich Schiller University Jena
  • Dr. Martin Roth
    Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (HKI)
  • Dr. Jörg Bossert
    Friedrich Schiller University Jena
  • Prof. Dr. Klaus D. Jandt
    Friedrich Schiller University Jena