Natural polymer based hydrogels are ideal scaffolds for tissue engineering1. Alginate based hydrogels have been widely used in tissue engineering because they are well known to be biocompatible2 and they possess many features similar to extra cellular matrix3. However, alginate hydrogels degrade very slowly and exhibit poor cell adhesion because of their lack of specific interaction with mammalian cells4. To overcome these drawbacks of alginate, several approaches are being investigated. For example, the oxidation of alginate can enhance its biodegradability, and the incorporation of specific proteins into the alginate based hydrogel can improve cellular interaction of the hydrogel. Keratin is a protein found as a main component in skin, hair, nail, hooves and horns. Keratins extracted from human hair and wool contain cell adhesive peptide sequences including RGD (arginine-glycine-aspartic acid), and LDV (leucine-aspartic acid-valine)5. The present study is aimed to prepare and to assess the properties of a novel type of hybrid hydrogel based on oxidized alginate and keratin protein extracted from wool. Oxidized alginate was synthesized via periodate oxidation of alginate. Keratin was extracted from wool by the treatment with urea, sodium metabisulfite and sodium dodecyl sulfate (SDS).The hybrid hydrogels were prepared using a sonication technique. The weight loss, protein release, FTIR, and mechanical property of the hydrogels were carried out. The results demonstrated that the composition of the hydrogels had a significant effect on their physical properties. Osteoblast-like MG-63 cells were grown on the hydrogels. Cell viability on the different hydrogels was compared by calcein fluorescent staining. The results indicated that the oxidized alginate/keratin hydrogels supported cell attachment and proliferation. The results proved that such novel hybrid hydrogels might be tuned to obtain materials suitable for tissue engineering applications.