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Poster Presentation

Multilayers of Nanoparticles with Alginate, Chondroitin sulfate and Hyaluronic acid for graded implants

Part of:
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- Poster Presentation PLA based CaCO3 composite fibers for textile scaffolds in hard tissue regeneration 1 Dr.-Ing. Claudia Hinüber
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- Poster Presentation Development and Characterization of Hybrid Hydrogel Based on Oxidized Alginate and Keratin for Tissue Engineering Applications 0 Supachai Reakasame
- Poster Presentation Nir Laser-responsive Folate-targeted Gold Nanorods As Efficient theranostic tool for Osteosarcoma Treatment 1 Ph.D. Nicol? Mauro
- Poster Presentation Mechanical properties of recent CAD/CAM polymer materials 1 Sebastian Spintzyk
- Poster Presentation Multilayers of Nanoparticles with Alginate, Chondroitin sulfate and Hyaluronic acid for graded implants 1 Steffen Sydow
- Poster Presentation Design of drug eluting ophthalmic lenses through a multi-layer approach 1 Dr. Ana Topete
- Poster Presentation Drug loaded silicon based soft contact lenses: effect of terminal sterilization methods on their performance 1 Andreia Sofia Oliveira
- Poster Presentation Investigating the compressibility of spray dried alumina-zirconia granules for use in orthopaedics - an easy to use and fast method 1 Dr. Ranko Adjiski
- Poster Presentation Biochemical modification of extruded protein nanofibers 1 Karsten Stapelfeldt
- Poster Presentation Microfibers and Nanoparticles with Controlled Dimensions of a Hyaluronic Acid Derivative 1 Ph.D. Calogero Fiorica
- Poster Presentation Modeling the Viscosity of Silicone Rubber During High-Speed Infrared Curing 1 Jan Stieghorst
- Poster Presentation Effect of the pH of bleaching H2O2 solutions in the enamel morphology, hardness and color 1 Dr. Ana Topete
- Poster Presentation New crosslinking diluents for dental materials 1 Prof. Dr. Norbert Moszner
- Poster Presentation Microstructure and Properties of Plasma Sprayed Bioactive Glass Ceramics Coatings 1 Annamária Dobrádi
- Poster Presentation Impact of chloride ions on apatite mineralisation on Bioglass® 45S5 0 Juliane Brandt-Slowik
- Poster Presentation In vivo performance of polymeric photodynamic active biomaterials for periodontal regeneration 1 Dr. Stefan Kranz
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- Poster Presentation Cellular interactions with extruded protein nanofibers 1 Naiana Suter
- Poster Presentation Antimicrobial efficacy of strontium and zinc glasses and glass ionomer cements against clinically relevant bacteria 0 Hawshan Mustafa
- Poster Presentation Mechanical properties of water-soluble polyvinyl alcohol parts fabricated by fused deposition modelling 1 Jochen Kuttig
- Poster Presentation Corrosion behavior of dental CoCr alloys made by different digital workflow processes compared to cast CoCr alloys 0 Christine Schille
- Poster Presentation Mechanical properties of biocompatible polymer-based resin for dental applications fabricated by stereolithography 1 Phan Hai-Binh Bui

Session P.2: Poster Session 2
Belongs to:
Topic P: Postersession

Functional implants between complex tissues are still an unsolved medical problem. Gradients along the implant could improve the situation at the tissue transition region. Besides gradients in the mechanical properties of the implant, the release of signaling proteins with spatial gradients would be a significant progress towards more functional implants. To generate such a gradient we use a combination of a nanoparticular drug release system and the Layer-by-Layer technology for immobilization. Biopolymers like chitosan or alginate can be used to prepare such nanoparticles for drug delivery applications. Our approach focusses on the incorporation of proteins, such as bone morphogenetic protein 2 (BMP-2) or transforming growth factor beta 3 (TGF-β 3) into the nanoparticles and the combination with polyelectrolytes such as alginate, chondroitin sulfate or hyaluronic acid in the LbL-technique to generate spatial gradients on a surface of an implant.

Nanoparticles were prepared by ionotropic gelation of chitosan and tripolyphosphate or alginate and Poly-l-lysine. The resulting particles were characterized via dynamic light scattering (DLS). Nanoparticle coatings were prepared by LbL using a dip-robot and alginate, chondroitin sulfate or hyaluronic acid as a polyanions. The resulting films were investigated by ellipsometry, atomic force microscopy (AFM) and scanning electron microscopy (SEM). Enzymatic degradation of the coatings was carried out at 37 °C in buffered solution and observed by ellipsometry.

Nanoparticles with a well-defined particle size and zetapotential have been obtained. The cationic nanoparticles were coated on silicon as a model surface by the LbL-method with different polyanions. A linear increase in layer thickness with the number dipping cycles is observed. Varying the dipping depth during the coating process resulted in a spatial concentration gradient on the silicon substrate. The degradation of the multilayer system has been proven by the decrease of the layer film thickness in lysozyme solution. BMP-2 was incorporated with high efficiency. Adapting the nanoparticle immobilization to electrospun fiber mats yields to promising results.


Steffen Sydow
TU Braunschweig
Additional Authors:
  • Henning Menzel
    Technische Universität Braunschweig