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Oral Poster

Recombinant Spider Silk Proteins Used as Biomedical Coating Material

Tuesday (09.05.2017)
18:39 - 18:42
Part of:
18:00 Oral Poster Fibrinogen Adsorption and Network Formation on Melt Drawn Isotactic Polybutene-1 Thin Films 1 Xiaoyuan Zhang
18:03 Oral Poster Characterization of biomaterials using AFM based fast nanoscale imaging and quantitative nanomechanical techniques 1 Dr. Tanja Neumann
18:06 Oral Poster Hydrothermal conversion of porous Ca carbonate biominerals into bioactive Ca phosphate materials: towards antibiotic and antiosteoporotic bone graft substitutes 1 Dr. Ingo Sethmann
18:09 Oral Poster Study on the retarding effect of phytic acid on the hydration of a brushite cement 1 Dr. Katrin Hurle
18:12 Oral Poster Synthesis of hollow mesoporous bioactive glass nanoparticles with uniform size and shape 1 Kai Zheng
18:15 Oral Poster Systematic post-operative clinical assessment of lumbar ventrolateral vertebroplasty in the large animal model sheep using a minimally-invasive approach 1 Francesca Gunnella
18:18 Oral Poster Dual setting system – characterization of PEG-based-hydrogel/brushite composites for biomedical application 0 Michaela Rödel
18:21 Oral Poster The effect of HAc etching on the degradation behaviour of Mg-5Gd 1 Dr. Frank Feyerabend
18:24 Oral Poster Degradation behavior of as cast and powder metallurgy processed Mg-Ca alloys 0 Dipl.-Ing. Eshwara Phani Shubhakar Nidadavolu
18:27 Oral Poster Bio-inspired high-performance green biopolymer fibers 0 Dr. Hendrik Bargel
18:30 Oral Poster Diatom Biosilica for Catalytic and Adsorptive Applications 1 Cathleen Fischer
18:33 Oral Poster Gallotannin polyphenol enhancement of biomimetic enzymatic hydrogel mineralization 1 Dr. Timothy E.L. Douglas
18:36 Oral Poster Starch in textile application: Modification, processing and product development 1 Pavan Kumar Manvi
18:39 Oral Poster Recombinant Spider Silk Proteins Used as Biomedical Coating Material 1 Christian Borkner
18:42 Oral Poster Effects of cross-linking modifications on Collagen: a preliminary biological evaluation 1 Dr. Mattia Vacchini
18:45 Oral Poster Endothelialization of polymethylpentene fibers as a step towards patient-specific medical devices 1 M.Sc. Annika Wenz
18:48 Oral Poster Hybrid poly-lactide/gelatin electrospun scaffolds with controlled hydrophilicity and wettability. 1 Germano Piccirillo
18:51 Oral Poster Funktion and structure - Combined optical functionality and specific bio-interaction for multifunctional biomedical materials 1 Dr. Tina Sabel
18:57 Oral Poster Bacterial adhesion to PEEK compared to exemplary dental ceramic, metallic and composite materials 1 Dr. Lutz Scheideler
19:00 Oral Poster Attachment of Nanoparticle Drug-Release Systems on Shish-Kebab structured Poly(?-Caprolactone) Nanofibers 1 Dominik de Cassan
19:06 Oral Poster Improved Nanofleeces for Cell Culture Applications 1 Tobias Kürbitz

Session P.1: Oral Poster Session 1
Belongs to:
Topic P: Postersession

Spider silk protein based materials are known for their good biocompatibility and biodegradability and lack of immunogenicity and allergenicity. For technical processing high amounts of proteins are required, but natural spider silk is rare. Biotechnological production of spider silk proteins gives rise to high yields and allows the genetic modification of the proteins.[1] The recombinantly produced and negatively charged spider silk protein eADF4(C16) is based on the consensus sequence of Araneus diadematus fibroin 4 (ADF4).[2] It contains 16 glutamatic acid residues which can be exchanged with lysine, yielding the positively charged eADF4(k16).[3] The proteins can be processed into films and coatings and the material properties can be adjusted to the desired processing method and fields of application.[1]

Common polymers for medical applications (implants, catheters) are polytetrafluoroethylene (PTFE), polyurethane (PU) and silicone. During application, the performance of biomaterials depends on the biocompatibility which is related to unwanted side effects (foreign body responses and inflammation) and the potential of interaction of cells with its surface. To avoid these problems, it’s important to adjust the material’s surface properties. The first successful application of eADF4(C16) coatings was shown on silicone breast implants to reduce periprosthetic fibrous capsule formation.[4]

The main objective of our research is to design and characterize novel spider silk coatings for biomedical applications. In the distinct application of catheters, low or even no cell adhesion is eligible. To influence the properties of the commonly used biomaterials and to increase their biocompatibility, a thin coating composed of eADF4(k16) acting as adhesion promoter and eADF4(C16) as outer layer was applied on PU, PTFE and silicone and evaluated regarding coating stability, degradation properties and cell adhesion.[5]

[1] C. B. Borkner et. al. ACS Appl. Mater. Interfaces 2014, 6, 15611.

[2] D. Huemmerich et. al. Biochemistry 2004, 43, 13604.

[3] E. Doblhofer & T. Scheibel J. Pharm. Sci. 2015, 104, 988.

[4] P. H. Zeplin Adv. Funct. Mater. 2014, 24, 2658.

[5] C. B. Borkner et. al. ACS Biomater. Sci. Eng. 2016, DOI: 10.1021/acsbiomaterials.6b00306.

Christian Borkner
University of Bayreuth
Additional Authors:
  • Dr. Stefanie Wohlrab
    Universität Bayreuth
  • Eva Möller
    Universität Bayreuth
  • Alexandra Pellert
    Universität Bayreuth
  • Prof. Gregor Lang
    Universität Bayreuth
  • Prof. Dr. Thomas Scheibel
    Universität Bayreuth